Monocyte chemotactic protein-1 gene and protein expression in atherogenesis of hypercholesterolemic rabbits
Section snippets
Experimental animals
Male New Zealand white rabbits (2 kg body weight) were fed with regular feed (control group) or regular feed containing 2% (wt/wt) cholesterol (Purina Mills, USA) for 1 day, 3 days, 1 week, 3 weeks or 6 weeks to induce hypercholesterolemia. During and after 6 weeks of feeding, serum total cholesterol and triglycerides were measured in blood samples collected from the ear vein. Four sets of cholesterol-feeding experiments were performed. In each set of experiments, each group consisted of two to
Plasma cholesterol and triglyceride levels
In the control group, the plasma cholesterol and triglyceride concentrations before the experiment was 0.70±0.064 g/l and 0.84±0.107 g/l, respectively (n=4). The concentrations did not change significantly during the 6-week feeding period (0.64±0.044 g/l and 0.83±0.046 g/l at 6-weeks for cholesterol and triglycerides, respectively, n=4). In the hyperlipidemic group, the plasma cholesterol concentration increased immediately 1 day after feeding cholesterol-containing chow from 0.73±0.072 g/l (n
Discussion
Hyperlipidemic rabbits fed with high-cholesterol diet are commonly used as experimental animals to examine the pathogenesis and mechanisms of atherosclerosis [33]. The atherosclerotic lesions observed in hyperlipidaemic rabbits are mainly composed of fatty streaks, similar to the type II lesions of human atherosclerosis [34]. In contrast to human atherosclerotic plaques which usually take decades to develop, the development of atherosclerosis in this animal model can be easily evaluated at
Acknowledgements
We thank Ya-Ting Chen and Shu-Feng Tsai for their assistance in taking photographs and W.L. Cheong for plotting figures. This work was supported in part by research grants from the National Science Council (NSC85-2331-B006-109M26 and NSC86-2314-B006-021M26) of ROC.
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