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Deoxyribonuclease I gene polymorphism and susceptibility to systemic lupus erythematosus

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Abstract

The DNASE1 gene is regarded as one of the susceptible genes for systemic lupus erythematosus (SLE). Recent studies have detected the presence of a variable number of tandem repeat (VNTR) polymorphisms at intron 4 in this gene. The current study aimed to investigate the influence of current polymorphism on SLE susceptibility in a sample of the Iranian population. The study included 163 patients and 180 unrelated healthy controls. The VNTR polymorphisms in the DNASE1 gene were determined by polymerase chain reaction (PCR). The genotypic frequency investigation indicated that 3/6 genotype frequency in patients affected with SLE was more than healthy controls (P = 0.004). Moreover, 3/4 and 4/6 genotype frequencies in healthy cohort were further in comparison with patient cohort (P = 0.0001). Findings of the present study manifested that 3/6 genotype in patients affected with SLE was significantly more than healthy controls, thus it can be regarded as a risk factor, while 3/4 and 4/6 genotypes were significantly higher in healthy controls which can be considered as a protective factor.

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Abbreviations

SEL:

systemic lupus erythematosus

DNASE1:

deoxyribonuclease I

VNTR:

variable number tandem repeat

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Acknowledgments

The authors thank the SLE patients and normal controls for participating in this study.

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Authors and Affiliations

  1. Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

    Milad Mohammadoo-Khorasani

  2. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Milad Mohammadoo-Khorasani

  3. Department of Biostatistics & Epidemiology, Hamadan University of Medical Sciences, Hamadan, Iran

    Mahsa Musavi

  4. Department of Biology, Zabol University, Zabol, Iran

    Mahdieh Mousavi

  5. Genomic Research Center, Birjand University of Medical Sciences, Birjand, Iran

    Maryam Moossavi

  6. Department of Clinical Biochemistry, Zahedan University of Medical Sciences, Zahedan, Iran

    Maryam Khoddamian

  7. Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Mahnaz Sandoughi & Zahra Zakeri

Authors
  1. Milad Mohammadoo-Khorasani
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  2. Mahsa Musavi
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  3. Mahdieh Mousavi
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  4. Maryam Moossavi
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  6. Mahnaz Sandoughi
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  7. Zahra Zakeri
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Correspondence to Maryam Moossavi.

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Mohammadoo-Khorasani, M., Musavi, M., Mousavi, M. et al. Deoxyribonuclease I gene polymorphism and susceptibility to systemic lupus erythematosus. Clin Rheumatol 35, 101–105 (2016). https://doi.org/10.1007/s10067-015-3111-y

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  • DOI: https://doi.org/10.1007/s10067-015-3111-y

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