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Lactobacillus casei reduces the Inflammatory Joint Damage Associated with Collagen-Induced Arthritis (CIA) by Reducing the Pro-Inflammatory Cytokines

Lactobacillus casei: COX-2 inhibitor

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Abstract

This study evaluated the therapeutic efficacy of Lactobacillus casei in treating rheumatoid arthritis using collagen-induced arthritis (CIA) animal model. Healthy female Wistar rats (weight—180–200 g) were included in this study. Oral administration of L. casei was started on the same day. Indomethacin was used as standard reference drug. Serum level of IL-6, α-TNF, and IL-10 were observed. Four-point arthritis indexes were also assessed at the end of week for 28th day. L. casei-treated rats had shown normal histopathology without any synovial infiltration, pannus formation, cartilage, and bone destruction. Arthritis score was also lower for the group treated with L. casei. Oral administration of L. casei significantly decreased the pro-inflammatory cytokines. Present study suggests that L. casei has potent antiarthritic effect in CIA model. Inhibition of COX-2 via inhibiting the pro-inflammatory cytokines is an understanding of the complex interactions involved in these pathways.

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Abbreviations

RA:

Rheumatoid arthritis

CIA:

Collagen-induced arthritis

CPCSEA:

Committee for the Purpose of Control and Prevention of Experiments on Animals

NSAIDs:

Non-steroidal anti-inflammatory drugs

DMARDs:

Disease-modifying anti-rheumatic drugs

LAB:

Lactic acid bacteria

IFA:

Incomplete Freund’s adjuvant

IL-6:

Interleukin-6

IL-10:

Interleukin-10

α-TNF:

Tumor necrosis factor

NF-κB:

Nuclear factor kappa

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Acknowledgements

We would like to acknowledge Madhya Pradesh Council of Science and technology (MPCoST) Bhopal, Madhya Pradesh, for providing financial support to conduct the study and Dr. Surendra Borkar for his valuable analysis of the histopatholgy. The author would like to thank Institute of Biomedical Sciences, Bundelkhand University, Jhansi, Uttar Pradesh for Animal House facility and Department of Microbiology, Barkatullah University, Bhopal for Laboratory facility provided to conduct the study.

Competing interests

There are nonfinancial competing interests (political, personal, religious, ideological, academic, intellectual, commercial, or any other) to declare in relation to this manuscript.

Author’s contribution

VS, RS, PS designed all experiment in this study and drafting of the manuscript. SA and PK handled animals and dosing protocols and other experiments. AK helped in statistical analysis.

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Authors and Affiliations

  1. Department of Microbiology, Barkatullah University, Hoshangabad Road, Bhopal, 462026, Madhya Pradesh, India

    Sarika Amdekar & Vinod Singh

  2. Institute of Biomedical Sciences, Bundelkhand University, Kanpur Road, Jhansi, 284001, Uttar Pradesh, India

    Rambir Singh

  3. Department of Zoology, Institute of Basic Sciences, Bundelkhand University, Kanpur Road, Jhansi, 284001, Uttar Pradesh, India

    Poonam Sharma & Poonam Keshav

  4. Veterinary Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makdoom, Mathura, 281122, Uttar Pradesh, India

    Avnish Kumar

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  1. Sarika Amdekar
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  2. Vinod Singh
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Correspondence to Vinod Singh.

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Amdekar, S., Singh, V., Singh, R. et al. Lactobacillus casei reduces the Inflammatory Joint Damage Associated with Collagen-Induced Arthritis (CIA) by Reducing the Pro-Inflammatory Cytokines. J Clin Immunol 31, 147–154 (2011). https://doi.org/10.1007/s10875-010-9457-7

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