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Contribution of immunofluorescence to identification and characterization of antineutrophil cytoplasmic antibodies in inflammatory bowel disease

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Abstract

We evaluated the combined use of different fixatives for the identification of atypical perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCA) in patients with inflammatory bowel diseases (IBD) by indirect immunofluorescence (IIF). Sera from 59 ulcerative colitis (UC) and 37 Crohn’s disease (CD) patients, and from 64 healthy controls were studied. The IIF on ethanol-, formalin-, and methanol-fixed neutrophils was used for the detection of ANCA. Enzyme linked immunosorbant assay (ELISA) was performed to identify the antigens recognized by ANCA. ANCAs were present in 35 of 59 (59.3%) UC patients and in 10 of 37 (27.02%) CD patients. Atypical p-ANCA positivity was strongly associated with UC disease (44.1% in UC vs. 8.1% in CD; p = 0.0002). The combined application of different fixatives contributed to make easy the differentiation between typical p-ANCA and atypical p-ANCA. Atypical p-ANCA determination appears to be a useful parameter for the distinction between UC and CD.

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Acknowledgements

This work was supported by Ministère de la recherché Scientifique et de la Technologie (MRST), Tunisia. We thank Mrs Samia Boukthir for her excellent technical assistance.

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Authors and Affiliations

  1. Immunology Department, Habib Bourguiba Hospital, 3029, Sfax, Tunisia

    Dorra Bouzid, Samy Haddouk, Ali Amouri, Youssef Ben Hadj Hmida, Nabil Tahri & Hatem Masmoudi

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  1. Dorra Bouzid
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  2. Samy Haddouk
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  3. Ali Amouri
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  4. Youssef Ben Hadj Hmida
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  5. Nabil Tahri
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  6. Hatem Masmoudi
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Correspondence to Dorra Bouzid.

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Bouzid, D., Haddouk, S., Amouri, A. et al. Contribution of immunofluorescence to identification and characterization of antineutrophil cytoplasmic antibodies in inflammatory bowel disease. Indian J Gastroenterol 30, 229–232 (2011). https://doi.org/10.1007/s12664-011-0127-3

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  • DOI: https://doi.org/10.1007/s12664-011-0127-3

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